野菊花总黄酮对肝纤维化大鼠治疗作用及机制探讨

毕明, 居靖, 吴繁荣*, 华传凤, 王珂, 董璇姣

中国药学杂志 ›› 2014, Vol. 49 ›› Issue (5) : 367-370.

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中国药学杂志 ›› 2014, Vol. 49 ›› Issue (5) : 367-370. DOI: 10.11669/cpj.2014.05.005
论 著

野菊花总黄酮对肝纤维化大鼠治疗作用及机制探讨

  • 毕明1, 居靖1, 吴繁荣2*, 华传凤2, 王珂2, 董璇姣2
作者信息 +

Therapeutic Effects and Mechanisms of Total Flavonoids of Chrysanthemum indicum on Liver Fibrosis in Rats

  • BI Ming1, JU Jing1, WU Fan-rong2*, HUA Chuan-feng2, WANG Ke2, DONG Xuan-jiao2
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摘要

目的 研究野菊花总黄酮对肝纤维化大鼠的治疗作用及机制。方法 SD大鼠随机分为正常对照组、模型组、野菊花总黄酮低剂量组、野菊花总黄酮中剂量组、野菊花总黄酮高剂量组和秋水仙碱阳性药对照组, 采用四氯化碳诱导大鼠肝纤维化模型, 造模后, 分别给予给药组相应的受试药物, 给予正常对常组和模型组生理盐水。观察野菊花总黄酮对肝纤维化大鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、透明质酸(HA)、层黏蛋白(LN)、Ⅲ型前胶原酶(PCⅢ)和Ⅳ型胶原酶(CⅣ)水平, 肝脏病理组织学及肝组织中胶原增生程度的影响;逆转录多聚酶链反应(RT-PCR)技术测定肝组织中转化生长因子-β1 mRNA的表达情况。结果 与模型组比较, 野菊花总黄酮组病理变化较模型组减轻, 野菊花总黄酮能显著降低大鼠肝组织羟脯氨酸以及血清中丙氨酸氨基转移酶、天冬氨酸氨基转移酶、透明质酸、层黏蛋白、Ⅲ型前胶原酶和Ⅳ型胶原酶含量, 野菊花总黄酮高、中剂量组明显抑制肝组织中转化生长因子-β1 的表达。结论 野菊花总黄酮对肝纤维化有明显治疗作用, 其机制可能与降低转化生长因子-β1 表达有关。

Abstract

OBJECTIVE To observe therapeutic effects and mechanisms of total flavonoids of Flos Chrysanthemum indicum(TFC) on liver fibrosis in rats. METHOD The model of rat liver fibrosis was adopted which was induced by CCl4 injection. The effects of TFC were observed on the levels of serum ALT, AST, HA, LN, PCⅢ and CⅣ in rats with liver fibrosis, and on liver histopathological changes as well as collagen hyperplasia formation in liver tissue. The gene expression of TGF-β1 was detected by RT-PCR. RESULTS Compared with the model group, TFC treatment significantly reduced ALT, AST, HA, LN, PCⅢ and CⅣ content in serum, liver histology in the TFC treated rats was also improved. Moreover TFC could decrease the expression of TGF-β1 .CONCLUSION TFC has a significant curative effect on the liver fibrosis rats, its mechanism probably be related to decrease the expression of TGF-β1.

关键词

野菊花总黄酮 / 肝纤维化 / 转化生长因子β1

Key words

total flavonoids of Flos Chrysanthemum indicum / liver fibrosis / transforming growth factor beta 1

引用本文

导出引用
毕明, 居靖, 吴繁荣*, 华传凤, 王珂, 董璇姣. 野菊花总黄酮对肝纤维化大鼠治疗作用及机制探讨[J]. 中国药学杂志, 2014, 49(5): 367-370 https://doi.org/10.11669/cpj.2014.05.005
BI Ming, JU Jing, WU Fan-rong*, HUA Chuan-feng, WANG Ke, DONG Xuan-jiao. Therapeutic Effects and Mechanisms of Total Flavonoids of Chrysanthemum indicum on Liver Fibrosis in Rats[J]. Chinese Pharmaceutical Journal, 2014, 49(5): 367-370 https://doi.org/10.11669/cpj.2014.05.005
中图分类号: R965   

参考文献

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基金

国家自然科学基金资助项目(81100301);安徽省自然科学基金资助项目(090413139)
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